Highly Infectious Prions Generated by a Single Round of Microplate-Based Protein Misfolding Cyclic Amplification
ABSTRACT Measurements of the presence of prions in biological tissues or fluids rely more and more on cell-free assays.Although protein misfolding cyclic amplification (PMCA) has emerged as a valuable, sensitive tool, it is currently hampered by its lack of robustness and rapidity for high-throughput purposes.Here, we made a number of improvements